Here, we report the identification of DIABLO, a proapoptotic protein that can directly interact with mammalian and baculoviral IAPs and interfere with MIHA-mediated UV protection presumably by dislodging active IAP bound caspases.
To identify proteins that bind mammalian IAP homolog A (MIHA, also known as XIAP), we used coimmunoprecipitation and 2D immobilized pH gradient/SDS PAGE, followed by electrospray ionization tandem mass spectrometry. DIABLO (direct IAP binding protein with low pI) is a novel protein that can bind MIHA and can also interact with MIHB and MIHC and the baculoviral IAP, OpIAP.
MIHA is an inhibitor of apoptosis protein (IAP) that can inhibit cell death by direct interaction with caspases, the effector proteases of apoptosis. DIABLO is a mammalian protein that can bind to
While no mammalian homologs of Grim, Reaper, or HID have been identified, because baculoviral and insect IAPs are able to inhibit Summary apoptosis in mammalian cells, their mechanism of action is likely to be conserved (Hay et al., 1995; Hawkins et To identify proteins that bind mammalian IAP homolog al., 1996).
The key effectors of apoptosis are caspases, cysteine proteases that cleave after aspartate residues. The inhibitor of apoptosis (IAP) family of proteins prevent cell death by binding to and inhibiting active caspases and are negatively regulated by IAP-binding proteins, such as …
Diablo homolog is a mitochondrial protein that in humans is encoded by the DIABLO gene on chromosome 12. DIABLO is also referred to as second mitochondria-derived activator of caspases or SMAC. This protein binds inhibitor of apoptosis proteins, thus freeing caspases to activate apoptosis. Due to its proapoptotic function, SMAC is implicated in a broad spectrum of tumors, and small molecule …
Aliases: DIABLO, DFNA64, SMAC, Diablo, Diablo homolog, diablo IAP-binding mitochondrial protein
AbstractTo identify proteins that bind mammalian IAP homolog A (MIHA, also known as XIAP), we used coimmunoprecipitation and 2D immobilized pH gradient/SDS PAGE, followed by electrospray ionization tandem mass spectrometry. DIABLO (direct IAP binding protein with low pI) is a novel protein that can
Identification of DIABLO, a mammalian protein that promotes apoptosis by binding to and antagonizing IAP proteins (Q28117928) From Wikidata. a mammalian protein that promotes apoptosis by binding to and antagonizing IAP proteins (English) 1 reference.
Jan 01, 2005 · Most pertinent here is Smac [second mitochondrial activator of caspases; known as DIABLO (direct IAP binding protein with low pI) in mouse], a nuclear encoded, mitochondrially localized protein which is released into the cytosol in response to apoptotic stimuli that disrupt the integrity of the mitochondria [7,8].
Verhagen et al. (2000) identified the murine homolog of SMAC, which they called DIABLO (direct IAP-binding protein with low pI). They showed that DIABLO can bind mammalian IAP homolog A (MIHA, or API3) and can also interact with MIHB (API1; 601712), MIHC (API2; 601721), and …
The possible gain of function of apoptosis could be mediated by the following mechanisms: (1) the DIABLO S71L mutant enhances the binding of XIAP-DIABLO; (2) the normal degradation of DIABLO is disrupted by the mutation, increasing the protein level of DIABLO S71L; or (3) the DIABLO S71L mutant enhances IAP auto-ubiquitination, increasing the